Welcome to the 3D Cell Electron Microscopy Core Facility
This facility houses a high-pressure freezer (Leica ICE) for preparation of biological samples for transmission electron microscopy, and a Talos F200C 200kV scanning transmission electron microscope with both cryo-EM and electron tomography capabilities. The facility specializes in electron tomography of cellular structures imaged at room temperature.
Usage Types:
Assisted: Staff can examine samples with or without the presence of the user.
Unassisted: A successfully trained user can examine samples without the presence of a staff member. Training is dependent on the instrument and the user’s competency as determined by the Core Manager.
Training: Usage of an instrument with specific intend to train a user for independent work.
Rates and Billing:
Academic users need to provide a funding string before booking. External clients can pay with a credit card.
Rates are determined based on usage type (assisted or unassisted) and user types (internal or external). Training is billed on assisted rates.
Billing will be done through iLab unless otherwise communicated. core page
| RATES 2025 | ||
| Talos F200C per hour | Room temp | Cryo |
| Internal Assisted | $125 | $140 |
| Internal Unassisted | $75 | $90 |
| External | $200 | $250 |
| High pressure freezer per shot | Leica ICE HPF | |
| Assisted Sapphire | $37 | |
| Unassisted Sapphire | $25 | |
| Assisted Metal | $32 | |
| Unassisted Metal | $20 | |
| Sectioning per hour | Leica UltraCut RT | |
| Own knife | $5 | |
| Facility knife | $50 | |
| Sample prep per sample | Internal | External |
| Substitution, Embedding, Sectioning, Staining | $250 | $300 |
Booking:
Staff are available from 9 am to 5 pm, Monday through Friday. Trained users can book instruments through iLab. https://uwmadison.ilabsolutions.com/landing/1802
Users who need assistance can book through staff. Please contact astrydom@wisc.edu.
The Cryo facility needs to be booked 48 h in advance to ensure enough liquid nitrogen is available and proper maintenance can be done after the session.
Cancelation:
No-shows will be billed for full scheduled appointment time.
If you need to cancel your reservation, delete your reserved time at least 24 hours in advance.
If you cancel within 24 hours, please contact Dr. Strydom (astrydom@wisc.edu) to communicate your cancellation directly.
Facility Director: Marisa Otegui, otegui@wisc.edu
Facility Manager: Amy Strydom, astrydom@wisc.edu
